This document is intended for thermo fisher scientific or authorized. The thermo scientific dionex ultimate 3000 vwd 3000 is a variable wavelength detector vwd series for industry leading uvvis detection. The picture below is a simulation at scale of the dionex ultimate 3000 hplc system on the lc ionbench table. Dionex ultimate 3000 series laboratory equipment pdf manual download. Standard operating procedure thermo scientific ultimate 3000. The thermo scientific ultimate 3000 basic manual system is optimized for easeofuse routine lc applications, and also offers uhplc compatibility with no loss in quality and reliability. Integrating hardware, software, and separation chemistry, we offer uhplc to everyonefor all needs. Dionex ultimate 3000 series operating instructions manual 236. Installation qualification manual for installation of the ultimate 3000 rslcnano. Dionex ultimate 3000 series thermo fisher scientific. Ultimate 3000 series flow managers thermo fisher scientific. For more information, refer to the ultimate 3000 rsclnano standard applications system manual.
Under the instruments tab bottom of navigation pane, the individual components. Operational qualification performance qualification for hplc. Ultimate 3000 basic manual system thermo fisher scientific. Operational qualification performance qualification for. Wps3000tsl analytical and wps 3000tsl semiprep wps3000tbsl analytical. Find the user manual and the help you need for the products you own at manualsonline. Covering flows from 20 nlmin to 10 mlmin and offering a wide range of pumping, sampling, and detection modules, the ultimate 3000 series provides solutions for all your chromatography needs. Dionex ultimate 3000 rslcnano standard applications. Ultimate 3000 uhplc and hplc manuals and quick installation. The descriptions in this manual apply to the following instruments in the ultimate 3000 acc3000 series. Document number document name for module language revision. The ultimate 3000 hplc series provides excellent chromatographic performance while maintaining easy and reliable operation.
It is 100% functional tested and ready to be operated. Thermo scientific dionex ultimate 3000 variable wavelength. The thermo scientific dionex coulochem iii detector provides utmost sensitivity for. Microfraction collection option for the ultimate 3000 series. Ultimate 3000 lc system provides a highly integrated solution with optimum fluidic connections, singlepoint and intelligent control through the chromeleon chromatography data. The dionex ultimate acc 3000 autosampler column compartments provides a costeffective solution for conventional lc and uhplc applications at up to 62mpa 9000 psi operating pressure. Dionex ultimate 3000 series dad3000mwd3000 service manual. Operating instructions manual dionex ultimate 3000 series operating instructions manual 236 pages. The thermo scientific ultimate 3000 basic manual system offers unique lc and entry level uhplc capabilities without compromising on quality and robustness. Ultimate 3000 scope the scope of this document is to present an overview of the high performance liquid chromatography hplc system ultimate 3000 and to provide the reader with guidelines for standard operations, routine maintenance and simple trouble shooting. From standard analytical applications to nano, rapid separation, uhplc configurations, and more. Dionex ultimate 3000 series manuals manuals and user guides for dionex ultimate 3000 series. Ultimate 3000 ncs3500rs and ncp3200rs thermo scientific. Dionex ultimate 3000 lpg3400a quaternary analytical pump.
The descriptions in this manual apply to the following instruments in the ultimate 3000 acc 3000 series. Operating instructions original operating instructions revision. The modular nature of the thermo scientific dionex ultimate 3000 platform does allow modifications to this layout. Dionex ultimate 3000 series dad3000mwd3000 service. Ultimate 3000 basic manual system abacus analytical systems. This system is the perfect choice for laboratories with moderate sample throughput for routine applications and also as a teaching system. The general ultimate 3000 rslcnano system overview is shown in figure 5. Ncs3500rs and ncp3200rs page 2 operating instructions tip. Detailed instrument description can be found in dionex. This makes it convenient to choose the detector that best optimizes sensitivity, specificity, and precision for your application requirements.
The layout of this manual is designed to provide quick reference to the sections of interest to the reader when operating the thermo scientific dionex ultimate 3000 pump. The ultimate 3000 hplc system offers uhplc compatibility across all modules, ensuring maximum performance for all users and all laboratories. Thermo scientific dionex ultimate 3000 products are uhplc compatible by design, establishing the new standard in conventional lc. Fullyscaleable, modular basic systems are designed to provide robust operation for consistent and reliable operation and are even compatible with uhplc applications up to 620 bar. Thermo scientific dionex ultimate 3000 series operating.
Therefore, the term the tcc is used throughout the manual. Hello, we have hplc thermo scientific dionex ultimate 3000 the problem is that the pressure becomes very high approximately 200 bar when we use the isoctratic mode using the analogy h20. Manuals and user guides for dionex ultimate 3000 series. Dionex ultimate 3000 rslcnano standard applications manual. The dionex ultimate 3000 rslc system is designed to deliver robust operation at high flow rates and high pressure. The ionbench lc table allows you to lift up or down easily your uhplc by commuting an electrical switch, for a convenient access to the top of your dionex ultimate 3000 hplc. Dionex ultimate 3000 the ultimate 3000 nano lc systems have been designed to optimize low flow separations and facilitate easy coupling to mass spectrometry to provide the best resolution, sensitivity, and selectivity for nano lc and proteomics applications. Pal user manual installation and operation gc pal pal gcxt. How do i change the deuterium lamp on a dionex ultimate 3000. Dionex recommends that you stack the individual modules of an ultimate 3000 system. Figure 2 starting chromeleon server please check the. Almost all descriptions in the manual apply to all configurations of the tcc3200. The ultimate 3000 detector family covers a wide range of detection. This document describes the standard setup, recommended experimental conditions and testing procedures to run standard applications on the thermo scientific dionex ultimate.
The basic manual system is perfect for teaching or low sample. If the module is part of an ultimate 3000 rslcnano system, see section 3. Thermo scientific dionex ultimate 3000 standard thermostatted column compartment tcc 3000 sd instruction manual as pdf via email request. Uv detectors dionex dad 3000 rs ultimate 3000 dionex mwd 3000 rs ultimate 3000 dionex vwd3100 ultimate 3000 dionex vwd3400rs ultimate 3000 dionex pda 3000 ultimate 3000 dionex pda100 dionex pda100u dionex ad25 dionex uvd 340u dionex uvd 170u dionex uvd 340s dionex uvd 170s dionex uvd 160s dionex uvd 320s. Thermo scientific dionex ultimate 3000 well plate autosampler. The forward optics design and wide range of available flow cells ensure optimal performance over a flow rate range of five orders of magnitude. Thermo scientific dionex ultimate3000 thermostatted column. The ultimate 3000 system is a costeffective solutions for conventional hplc and uhplc. Ionbench lc with the dionex ultimate 3000 hplc system. Almost all descriptions in the manual apply to the following variable wavelength detectors in the ultimate 3000 series. Ics2100 ion chromatography system operators manual. Detailed instrument description can be found in dionex reference library cdrom which contains all relevant and specific manuals. If the lamp on your dionex ultimate 3000 series hplc system fails, follow the instructions below to replace your deuterium lamp.
The basic manual system is perfect for teaching or low sample throughput, routine applications. Dionex was acquired in 2010 by thermo fisher scientific. The descriptions in this manual apply to the following autosamplers in the ultimate 3000 autosampler series. Document number, document name, for module, language, revision.
Vwd3100 vwd3400rs the following conventions apply to the descriptions throughout this manual. Micro fraction collection option for the thermo scientific dionex ultimate. The unit is in excellent cosmetic as well as working condition. Lpg3400xrs pump operating instructions page 1 1 introduction 1. Standard operating procedure thermo scientific ultimate. In the chromeleon console, verify that the instrument is connected to the software. Instrument overview the dionex ultimate 3000 system is a dual pump system with three channels on each pump. We have 1 dionex ultimate 3000 series manual available for free pdf download. Dionex vwd3400rs ultimate 3000 dionex pda 3000 ultimate 3000 dionex pda100 dionex pda100u dionex ad25 dionex uvd 340u dionex uvd 170u dionex uvd 340s dionex uvd 170s dionex uvd 160s.
However, in order to obtain a full understanding of your thermo scientifica. However, in order to obtain a full understanding of your thermo scientific. The deuterium lamp emits uv radiation that is harmful to the eyes when switched on. View and download thermo scientific dionex ultimate 3000 series operating instructions manual online. This manual is intended only for dionex or authorized service. Vwd3100 and vwd3400rs variable wavelength detectors operating instructions page 1 1 introduction 1. It integrates an autosampler and column compartment with optional sample thermostatting in a single module and offers performance and flexibility that exceed. Sd, rs, bm, and bx pumps operating instructions page 1 1 introduction 1.
This manual is intended only for dionex or authorized service personnel to install the micro fraction collection option in the ultimate 3000 series nanocap autosamplers wps 3000 tb and should not be made available to other individuals. The thermo fisher dionex ultimate 3000 series lpg3400a quaternary analytical pump is a pump 4 solvents with a integrated vacuum degasser and mixing chamber. Highquality autosampler components, intelligent mechanical and electronic design. Rslcnano system overview srd3400, srd3200 with degassing or sr3000 without degassing. Ultimate 3000 rslcnano standard applications 4 1 introduction this document describes the standard setup, recommended experimental conditions and testing procedures to run standard applications on the thermo scientific dionex ultimate 3000 rslcnano system. Dionex ultimate 3000 basic manual hplc system in markham, ontario, canada. Open the purge valve, by opening the all the scientific staff members carrying out the ultimate 3000 pump compartment a hplc make dionex, model ultimate metal screw is located, loose it rotate it 3000 are responsible for strictly adhering to the in the anticlockwise direction. Wps 3000 tsl analytical and wps 3000 tsl semiprep wps3000tbsl analytical. The dionex ultimate 3000 comes in a variety of lc system configurations. Operating instructions manual dionex ultimate 3000 series operating instructions manual 236 pages sd, rs, and bm pumps. I think the last time they were maintained by dionex the wash systems decided to behave but with this new information i may be able to ge tthem to repairreplace the motor. Enjoy cost effective access to uhplc separations in a simple, expandable system where modularity makes anything possible. View and download dionex ultimate 3000 series operating instructions manual online. Tcc3200 column compartment page 2 operating instructions 1.
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